Figure 6

Effect of bleomycin on lung fibrotic and biochemical changes in wild-type and CD69-deficient mice. (A) Ashcroft scores, which are a semi-quantitative measure of lung fibrotic changes, were determined 14 d after the instillation of BLM or PBS (sham treatment). Please see the Methods section for an explanation of the scoring criteria. Results are expressed as the mean (SEM) (n = 4 mice per group). *P < 0.01 vs. sham-treated mice. †P < 0.01 vs. WT mice. (B) The lung collagen content was measured 14 d after the instillation of BLM or PBS (sham treatment). Results are expressed as mean (SEM) (n = 6-8 BLM-treated mice, n = 3 sham-treated mice). *P < 0.01 vs. sham-treated mice. †P < 0.01 vs. WT mice. (C) The mRNA expression level of TGF-β1 in the lung was measured 7 d after the instillation of BLM or PBS in WT or CD69KO mice.