Granuloma composition in cathepsin K-deficient mice. (A), granuloma with several foreign body-type MGCs. (B), granuloma with Langhans-type MGC (C), cathepsin K-deficient mice had significantly higher number of MGCs per lung section, (* - p < 0.05 when compared to Apoe-/- mice). (D), Mac-3 positive cells in granuloma (green - Mac-3, blue - nuclei), (E), osteopontin-containing epitheloid and giant cells (red - osteopontin, blue - nuclei), (F), merge of D and E. (G), percentage of granuloma area stained for Mac-3, osteopontin, CD4 and CD8. (H) The collagen-stained area was quantified using color threshold and calculated as percentage of granuloma area (* - p < 0.05 when compared to Apoe-/- mice). (A, D-F), scale bars 30 μm, (B), scale bar, 20 μm.