Figure 5

Co-stimulatory molecule expression in wild type and PAR-2-deficient mice following exposure to GC frass. BALB/c and PAR-2-/- mice were administered a single intratracheal inhalation of PBS (40 μl) or GC frass (40 μg/40 μl) and whole lungs were isolated 20 h later. Cells were dissociated from the lungs, and stained for flow cytometry analysis. mDC populations were analyzed for CD86 and CD80 expression. Mean fluorescent intensity (MFI) of CD80 (A) and CD86 (B) and percentage of cells expressing CD80 (B) and CD86 (D) are shown. Values are means ± SEM, n = 8 mice/group and statistical analysis was performed by ANOVA (*p < 0.001, **p < 0.05).