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Figure 1 | Respiratory Research

Figure 1

From: Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia

Figure 1

Design of SPC-sICAM-1 transgene construct and transfection into MLE12 cell line. The transgene, sICAM-1, was placed under the control of the human SPC promoter (-3700 to +24 bp). The SV40 cassette provided intronic and polyadenylation sequences. The approximate locations of primers for genotyping transgenic mice are indicated (arrowheads) together with the PCR product (a). Function of the transgene was demonstrated by transient transfection of the transgene into MLE12 cells (b). sICAM-1 in the cell culture supernatants was measured at 24 and 48 hrs as shown (n = 3, * P < 0.05).

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Respiratory Research

ISSN: 1465-993X

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