Figure 6

Immunofluorescence staining of human pulmonary microvascular ECs (HPMVECs) and endothelial-like cells (ELCs) for the autophagic marker, LC3 (A-C), mitochondrial marker mitotracker red (D-F), and SOD-2 (G-I). A), HPMVECs co-cultured with PAFLCs; B), with MFLCs; C), with MFLCs + Rapamycin; A) and C), The formation of autophagosomes (green punctate structures) was detected (see inset). D), HPMVECs co-cultured with PAFLCs; E), MFLCs; F), with MFLCs + Rapamycin; D) and F), The normal filamentous mitochondrial reticulum (red punctate structures) was detected (see inset). G), HPMVECs co-cultured with PAFLCs; H), with MFLCs; I), with MFLCs + Rapamycin; G) and I), SOD-2 expression (green punctate structures) was detected (see inset). The blue staining was DAPI. The magnification was 400×. Scale bar = 25 μm. ELCs = endothelial-like cells.