Figure 4
From: Hepcidin expression in human airway epithelial cells is regulated by interferon-γ
Fpn surface expression is unchanged in the presence of exogenous hepcidin or IFN-γ. NHBE cells, grown at ALI, were pre-treated with either IFN-γ for 24 hours prior or exogenous hepcidin peptide (1 μM) for 2 hours prior to biotinylation of cell surface proteins and cell harvest. Equal quantities of protein (300 μg) were separated by SDS-polyacrylamied gel electrophoresis (12%) followed by immunoblotting using an Fpn antibody (1:1000, generously provided by J. Kaplan, University of Utah) and standardized to Coumassie staining.