Figure 5

Activation of p38 MAPK after PCBG stimulation of 1HAEo-cells. A. 1HAEo^- cells were incubated with 100 μg/ml of PCBG for a period of eight hours, and the media supernatants collected and IL-8 measured by ELISA. Prior to the addition of PCBG the cells were pretreated for 1 hour with the p38 inhibitor SB202190. Data were analyzed with one-way ANOVA and posttest Bonferroni comparison (***denotes p < 0.001). B. 1HAEo^- cells were challenged with 100 μg/ml of PCBG for the times indicated and phospho-p38 and total p38 analyzed by western blot in the total cell lysates. C. Densitometry analysis of phospho-p38 to total p38 ratio. The data shown is representative of three independent experiments.