Figure 2
H 4 R antagonism decreases antigen-specific lymph node proliferation and cytokine production. (A) PBLN from vehicle, JNJ 7777120 (20 mg/kg) and anti-IL-13 treated animals were cultured with and without the addition of ovalbumin. Proliferation was determined by the measurement of incorporated 3H thymidine. (B) Supernatants from parallel lymph node cultures treated with ovalbumin were assayed for IL-4, IL-5 and IL-13 by ELISA. Lymph nodes were pooled from 8-10 animals per group and assayed in quadruplicate. Significance of each treatment group compared to control vehicle-treated animals is as follows: * P < 0.05, ** P < 0.01, *** P < 0.001, Ψ < 15 pg/ml, the lower limit of quantification in this assay.