Table 1 Real-time primer sequences and basal levels of transcript expression in normal human lung fibroblasts
GenBank Identifier | Gene | Forward Primer | Reverse Primer | Basal Ct |
|---|---|---|---|---|
NM_001105 | ALK-2 | CGGGAGATGACCTGTAAGACCCCG | GGGCCGTGATGTTCCTGTTAC | 25.00 ± 0.70 |
NM_004329 | ALK-3 | CAGAAACCTATTTGTTCATCATTTCTCG | ATCCCAGTGCCATGAAGCATAC | 21.97 ± 0.82 |
NM_001203 | ALK-6 | CGAATGGGGTGTAGGTCTTTATTACATTCG | CCCATTCCTCATCAAAGAAGATCA | 26.50 ± 0.93 |
NM_001204 | BMPRII | CGGTTTCCACCTCATTCATTTAACCG | ACAGAGACTGATGCCAAAGCAAT | 24.93 ± 0.42 |
NM_000088 | COL1a1 | CTTTGCATTCATCTCTCAAACTTAGTTTT | CCCCGCATGGGTCTTCA | 19.03 ± 0.69 |
NM_001845 | COL4a1 | CTAATCACAAACTGAATGACTTGACTTCA | AAATGGCCCGAATGTGCTTA | 19.87 ± 0.95 |
X02761 | Fibronectin | TGGACCAGAGATCTTGGATGTTC | CGCCTAAAACCATGTTCCTCAA | 21.70 ± 0.79 |
X56160 | Tenascin C | GGTCCACACCTGGGCATTT | TTGCTGAATCAAACAACAAAACAGA | 17.00 ± 0.92 |
NM_001613 | αSMA | CCGACCGAATGCAGAAGGA | ACAGAGTATTTGCGCTCCGAA | 20.60 ± 0.10 |
NM_021009 | UBC | CACTTGGTCCTGCGCTTGA | TTTTTTGGGAATGCAACAACTTT | 17.50 ± 1.35 |
NM_012423 | RPL13A | CCTGGAGGAGAAGAGGAAAGAGA | TTGAGGACCTCTGTGTATTTGTCAA | 19.65 ± 0.31 |
NM_004168 | SDHA | TGTGTCCATGTCATAACTGTCTTCATA | AAGAATGAAGCAAGGGACAAAGG | 19.00 ± 0.91 |