Table 2 Antibodies used for flow cytometry
From: Enhanced effector function of cytotoxic cells in the induced sputum of COPD patients
Antigen | Fluorochrome | Isotype | Clone | Source |
|---|---|---|---|---|
CD3 | ECD PC7 | Mouse IgG1 | UCHT1 | Beckman Coulter, Luton, UK |
CD4 | FITC PC5 | Mouse IgG1 | 13B8.2 | Beckman Coulter, Luton, UK |
CD8 | PC5 APC | Mouse IgG1 | B9.11 | Beckman Coulter, Luton, UK |
CD8 | ECD | Mouse IgG1 | SFCl21Thy2D3 | Beckman Coulter, Luton, UK |
CD16 | PC7 | Mouse IgG1 | 3G8 | Beckman Coulter, Luton, UK |
CD19 | PC5 | Mouse IgG1, k | J4.119 | Beckman Coulter, Luton, UK |
CD45RA | FITC PE | Mouse IgG1 | ALB11 | Beckman Coulter, Luton, UK |
CD45RO | ECD | Mouse IgG2a | UCHL1 | Beckman Coulter, Luton, UK |
CD56 | PE PC5 PC7 | Mouse IgG1 | N901 | Beckman Coulter, Luton, UK |
CD62L | PC5 | Mouse IgG1 | DREG56 | Beckman Coulter, Luton, UK |
Granzyme B | FITC | Mouse IgG1k | GB11 | Becton Dickinson, Oxford, UK |
Perforin | PE | Mouse IgG2b | (G9 | Becton Dickinson, Oxford, UK |
CXCR3 | PE | Mouse IgG1, k | 1C6/CXCR3 | Becton Dickinson, Oxford, UK |
VLA-4 | FITC | Mouse IgG1 | HP2/1 | Beckman Coulter, Luton, UK |