Figure 2
Verapamil reduces short-circuit (Isc) level in H441 monolayers in a dose-dependent manner. (A) Representative Isc trace showing applications of a series of concentrations. Amiloride-sensitive Isc level (AS Ibasalsc) is the sum of verapamil-inhibitable and residual amiloride-sensitive fractions. (B) Normalized AS Isc levels (AS Iverasc/AS Ibasalsc) at each concentration were plotted as a dose-response curve. n = 6. The raw data were fitted with the Hill equation. IC50 value, 294.2 μM. (C) & (D) Verapamil on amiloride-insensitive Isc levels in amiloride pretreated cells. Representative trace (C) and corresponding average Isc levels before (Control) and after addition of amiloride and verapamil (D). n = 3. P = 0.89 for the Isc levels before and after verapamil. Paired t-test.