Figure 7From: Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesisDe novocholesterol synthesis in CF mouse lung compared to matched controls. A) Deuterium incorporation into newly synthesized tissue cholesterol was measured by GC/MS. Data is normalized to each tissue wt control (represented by dotted line) and reported as fold increase of % newly synthesized cholesterol/8 h. Filled bars represent ΔF/ΔF mice and open bars represent R/R mice. The number of replicates is shown in parenthesis above each bar and represents individual assay on multiple tissue samples obtained over 3 experiments. *p < 0.05, #p < 0.01. B) Increased SRE response in CFTRinh-172 treated control epithelial cells. 9/HTEo-pCEP (wt) cells were incubated in serum free conditions for 24 h with or without 20 μM CFTRinh-172 (INH-172) in serum free media for an additional 24 h. 9/HTEo-pCEP are open bars. Data are normalized to serum free NT control levels over 3 experiments. Number (n) of samples is in parenthesis above each bar. Significance was determined by t test. Error bars represent SEM. * p < 0.0001.Back to article page