Figure 7
From: Cigarette smoke and lipopolysaccharide induce a proliferative airway smooth muscle phenotype
CSE and LPS induce ERK 1/2 and p38 MAP kinase phosphorylation in BTSM strips. BTSM strips were cultured for 8 days with or without LPS (1 μg/ml) or exposed to 15% CSE for 1 h daily during 8 days. Tissue lysates were analyzed by immunoblotting for phospho-ERK 1/2 (Thr202/Tyr204) and phospho-p38 MAP kinase (Thr180/Tyr182). Differences in protein loading were corrected for by immunoblotting for total ERK 1/2 and total p38 MAP kinase. Immunoblots were quantified using densitometry. The abundance of CSE- and LPS-induced ERK 1/2 and p38 MAP kinase phosphorylation was normalized to controls from untreated strips. Data represent means ± S.E.M. of 3-6 experiments. *P < 0.05, **P < 0.01 vs control