Figure 7
Effect of T0901317 on carrageenan-induced Bax and Bcl-2 expression in the lung. Representative Western blots showing no Bax expression in lung tissues obtained from sham-treated animals (Ae, Ae1). Bax levels were increased in the lung tissues from carrageenan-treated mice (Ae, Ae1). T0901317 (20 mg/kg) treatment prevented the carrageenan-induced Bax expression (Ae, Ae1). A basal level of Bcl-2 expression was detected in lung tissues from sham-treated mice (Be, Be1). At 4 hours after carrageenan administration, Bcl-2 expression was significantly reduced (Be, Be1). Treatment of mice with T0901317 (20 mg/kg) significantly attenuated carrageenan-induced inhibition of Bcl-2 expression (Be, Be1). A representative blot of lysates obtained from 5 animals per group is shown and densitometry analysis of all animals is reported. The results in panel Ae1 and Be1 are expressed as mean ± s.e.m. from n = 5/6 lung tissues for each group. *P < 0.01 versus sham group. °P < 0.01 versus carrageenan. Lung sections taken from carrageenan-treated mice pre-treated with vehicle showed positive staining for Bax (Ab, Ad) localized mainly in the inflammatory cells. The degree of positive staining for Bax was markedly reduced in lung sections obtained from mice pre-treated with 20 mg/kg T0901317 mice (Ac, Ad). Positive staining for Bcl-2 was observed in lung sections taken from sham mice (Ba, Bd). The degree of positive staining for Bcl-2 was markedly reduced in lung sections obtained from carrageenan-mice treated with vehicle (Bb, Bd). Pre-treatment with T0901317 20 mg/kg significantly attenuated the reduction in Bcl-2 expression caused by carrageenan (Bc, Bd). The figure is representative of at least 3 experiments performed on different experimental days. ND: not detectable Data are expressed as mean ± s.e.m. from n = 10 mice for each group. *P < 0.01 versus sham group. °P < 0.01 versus carrageena.