Figure 5
From: JNK activation is responsible for mucus overproduction in smoke inhalation injury
Smoke-induced JNK activation. Western blotting performed with an antibody that recognizes the phosphorylated form of JNK. Mice were exposed to cotton smoke for 15 min, which was followed by a recovery period of 4 and 24 h. JNK inhibitor (SP-600125) treated and JNK -/- mice did not show pJNK protein expression after smoke inhalation. Immunofluorescence (IF) showing JNK activation (D-F) in response to smoke. Green, phosphorylated JNK; blue, nuclei (4'-6-diamidino-2-phenylindole (DAPI). (Magnification, 400×). Wild-type control group mice did not show expression of pJNK. pJNK activation was observed predominantly in the small airway epithelium of the mice subjected to smoke inhalation at 4 and 24 h after recovery. A, WC DAPI; B, WS4 DAPI; C, WS24 DAPI; D, WC JNK IF; E, WS4 JNK IF; F, WS24 JNK IF.