Figure 4

Pulmonary and blood eosinophil recruitment. Bronchoalveolar lavage eosinophils (A) and lung homogenate eosinophil specific peroxidase (EPO) activity (B) were measured in CRA fed and PBS fed mice 4 hours after final CRA challenge. EPO was assessed in the lung homogenate following lavage to collect cells. Representative cytospin images from CRA fed (C) and PBS fed (D) mice stained with H+E at 100×. The circulating blood levels of eosinophils (E) were assessed in CRA fed and PBS fed mice 4 hours after final challenge using a Hemavet. Each value is the mean ± SEM for n = 18. ** = p < 0.01 comparing CRA fed to PBS fed mice.