Figure 10
From: Modulation of cytokine and nitric oxide by mesenchymal stem cell transfer in lung injury/fibrosis
Localization of the Y chromosome in bleomycin (BLM)-treated rats following bone marrow-derived mesenchymal stem cell transfer. A 2-μm-thick section of lung tissue from a BLM-treated rat hybridized with a fluorescein isothiocyanate-conjugated Y chromosome paint probe and counterstained with ethidium bromide is shown. (A) Bleomycin treated rats (n = 3). (B) Bone marrow-derived mesenchymal stem cell in recipient lungs under fluorescent microscope two weeks after BMDMSC injection in BMDMSC transfer rats (n = 3). The arrows indicate nuclei containing a Y chromosome. Magnification = 1000×. (C) Donor cells containing the Y chromosome zoomed out pictures of the lung. Magnification = 400×. (D) Localization of the cells containing the Y chromosome in serial section of lung tissue of BMDMSC transfer rats. H&E stain, magnification = 400×.