Figure 4

IFN-γ increases whereas IL-13 decreases proSP-C. Adult human ATII cells were cultured on Matrigel and rat-tail collagen coated inserts in DMEM containing 5% heat inactivated human serum for 2 d with10 ng/ml TGFα followed by 4 d 10 ng/ml KGF with or without 4 d 20 ng/ml IL-13 or 100 ng/ml IFN-γ. Panel A shows protein levels by immunoblotting for four different proSP-C bands normalized by GAPDH from 6 different donors cells stimulated by 4 d 20 ng/ml IL-13 or 100 ng/ml IFN-γ, which are analyzed by NIH Image. Representative protein levels by immunoblotting are shown on Figure 1 Panel B lane 3, 5 and 7. White bar: without IL-13 and IFN-γ, black bar: with 20 ng/ml IL-13 for 4 d, grey bar: with 100ng/ml IFN-γ for 4 d. *: p < 0.05 v.s without IL-13 or IFN-γ. Panel B shows immunocytochemistry for proSP-C. Top picture: without IL-13 or IFN-γ, middle one: with IL-13, bottom one: with IFN-γ. green: proSP-C, blue: DAPI. These three pictures were taken at the same exposure times.