Figure 2
From: SPLUNC1 regulation in airway epithelial cells: role of toll-like receptor 2 signaling
Effects of Mycoplasma pneumoniae (Mp) and a TLR2 agonist (Pam3CSK4) on SPLUNC1 production in cultured mouse tracheal epithelial cells. Tracheal epithelial cells from TLR2+/+ and TLR2-/- mice on the BALB/c background were isolated and cultured under the air-liquid interface (ALI) conditions for 10 days as described in the Methods section. After 48 hrs of Mp (10 cfu/cell) or Pam3CSK4 (1 μg/ml) treatment, SPLUNC1 protein levels in apical supernatants of tracheal epithelial cells were measured using Western blot, quantified using densitometry, and normalized to non-treated (-) cells to obtain SPLUNC1 protein relative levels. As compared to the non-treatment control (-), Mp or Pam3CSK4 treatment in tracheal epithelial cells from TLR2+/+, but not TLR2-/- mice, significantly increased SPLUNC1 protein levels. Data are expressed as means ± SEM (N = 3-4 replicates).