Figure 4

Levels of peribronchial fibronectin in Siglec-F deficient vs WT mice. Different groups of Siglec-F deficient or WT mice were subjected to chronic OVA challenge. Non-OVA challenged mice served as a control. Lung sections were immunostained with an anti-fibronectin Ab and the area of peribronchial fibronectin immunostaining determined by image analysis using a light microscope objective at 20× (Figure 4 A-E). Chronic OVA challenge in WT mice induced a significant increase the area of peribronchial fibronectin immunostaining (p < 0.0001*)(Fig 4A; Fig 4B vs Fig 4D)(WT no OVA vs WT OVA). The area of peribronchial fibronectin immunostaining was also significantly increased in OVA challenged Siglec-F deficient compared to OVA challenged WT mice (p < 0.01^#)(Fig 4A; Fig 4E vs Fig 4D)(n = 16 mice/group).