Figure 7
Nicotine dampens ATP-induced increase in [Ca2+] i . (A) BAL cells were stimulated with ATP (10-4 M) in the presence or absence of nicotine (10-4 M) or epibatidine (10-6 M). * P ≤ 0.01 (nicotine compared to Hepes) (B) The nicotine-induced effect is blocked by pre-incubation with the nicotinic antagonist α-bungarotoxin (10-7 M). ***P ≤ 0.001 (nicotine compared to α-bungarotoxin + nicotine) #P ≤ 0.05 (Hepes compared to α-bungarotoxin + nicotine). (C) Nicotine-mediated effect on ATP-induced [Ca2+]i rise is not depended on extracellular calcium. BAL cells were treated with nicotine (10-4 M) or with the vehicle in the presence of extracellular Ca2+. Immediately before measurements, cells were transferred to Ca2+-free buffer where a constant leakage of Ca2+ from the cells can be observed. BAL cells were pre-treated with nicotine or with the vehicle, and then stimulated with ATP (10-4 M). Number of cells which reacted, and total number of measured cells are given in brackets. ***P ≤ 0.001, *P ≤ 0.05.