Figure 5
Patch clamp recordings on AM (A, B, D-F) and on PC12 cells (C). (A) Representative whole cell recording depicting the measured membrane current (IM) at -60 mV in absence of and following ATP application (2 × 10-4 M, indicated by arrow). Agonist was applied directly to the bath via a pipette. ATP activates transient inward currents. (B) Control recording where bath solution was applied instead of agonist. (C) Effect of ACh (10-4 M) on PC12 cells. ACh induces strong inward currents. (D) Application of ACh to the bath solution has no effect on membrane currents in AM. Insert: The use of a perfusion system also did not induce any significant changes of the membrane current in response to ACh. (E, F) Current-voltage relationship recorded in the absence of (control, E) and immediately after ACh application (F). In these recordings, again no changes in membrane current were detected.