Incubation time | Nicotine (μM) |  | 5-HT |  | Acetylcholine | ||
---|---|---|---|---|---|---|---|
 |  | n | E max (mN) | pEC 50 | n | E max (mN) | pEC 50 |
Day 1 | 0 (Ctrl) | 9 | 1.87 ± 0.32 | 6.47 ± 0.13 | 8 | 5.81 ± 0.74 | 6.51 ± 0.12 |
 | 10 | 10 | 1.97 ± 0.26 | 6.45 ± 0.10 | 8 | 6.20 ± 0.62 | 6.46 ± 0.07 |
Day 2 | 0 (Ctrl) | 11 | 2.01 ± 0.29 | 6.83 ± 0.09 | 8 | 6.45 ± 0.70 | 6.57 ± 0.06 |
 | 10 | 12 | 1.99 ± 0.31 | 6.87 ± 0.09 | 8 | 5.95 ± 0.73 | 6.56 ± 0.10 |
Day 4 | 0 (Ctrl) | 10 | 2.01 ± 0.23 | 6.98 ± 0.08 | 6 | 6.04 ± 1.05 | 6.43 ± 0.07 |
 | 1 | 9 | 1.89 ± 0.28 | 7.00 ± 0.13 | 6 | 5.24 ± 0.64 | 6.56 ± 0.12 |
 | 10 | 8 | 1.88 ± 0.18 | 6.89 ± 0.18 | 6 | 5.70 ± 0.49 | 6.61 ± 0.11 |