Ovalbumin sensitization and challenge increases the number of lung cells possessing a mesenchymal stromal cell phenotype

Table 2 Selected genes upregulated in sorted lung MSCs relative to lung fibroblasts.

Symbol average	Lung MSCs average	Lung fibroblast	Fold difference	Differential p-value
Secreted cytokines and paracrine factors
Prl2c3	20800	2.91	7147.77	1.00E-13
Prl2c4	16700	2.49	6706.83	1.01E-13
Prl2c2	16400	2.37	6919.83	1.01E-13
Efnb2	250	42.4	5.90	2.98E-08
Prl2c5	224	71.5	3.13	7.15E-08
Grem1	17700	2520	7.02	1.42E-06
Ptges3	2080	763	2.73	1.20E-05
Fgf10	3710	853	4.35	0.000233
Ctf1	265	109	2.43	0.000226
Ccl25	171	58.9	2.90	0.000239
Fgf7	7600	3580	2.12	0.00038
Efna4	176	85.3	2.06	0.00268
Bmper	2060	1020	2.02	0.0041
Lefty1	411	244	1.68	0.0163
Gdf11	52.3	7.81	6.70	0.0279
Thbs1	961	300	3.20	0.0293
Cx3cl1	384	81.6	4.71	0.0311
Sema3a	638	14.8	43.11	0.0367
Spp1	12500	8920	1.40	0.0469
TGF-β1 signaling
Smad2	334	86.4	3.87	5.67E-07
Smad3	889	410	2.17	5.67E-07
Mapk13	387	104	3.72	1.28E-06
Hdac2	2440	1300	1.88	1.58E-05
Trrap	207	54.2	3.82	4.02E-05
Hdac4	179	69.6	2.57	0.000433
Mapk11	115	30.3	3.80	0.000661
Tgfbrap1	603	195	3.09	0.00172
Nfatc3	287	121	2.37	0.00226
Hdac3	1080	377	2.86	0.00241
Hdac10	60.3	19.4	3.11	0.00378
Acvr2b	177	107	1.65	0.0308
Endogl1	77.5	39.1	1.98	0.0462

Abbreviations are: Prl2c3, encoding prolactin family 2, subfamily c, member 3; Prl2c4, prolactin family 2, subfamily c, member 4 ; Prl2c2. prolactin family 2, subfamily c, member 2; Efnb2, ephrin B2; Prl2c5, prolactin family 2, subfamily c, member 5; Grem1, gremlin 1; Ptges3, prostaglandin E synthase 3; Fgf10, fibroblast growth factor 10; Ctf1, cardiotrophin 1; Ccl25, chemokine (C-C motif) ligand 25; Fgf7, fibroblast growth factor 7 or keratinocyte growth factor; Efna4, ephrin A4; Bmper, BMP-binding endothelial regulator; Lefty1, left right determination factor 1; Gdf11, growth differentiation factor 11 (BMP 11); Thbs1, thrombospondin 1; Cx3cl1, chemokine (C-X3-C motif) ligand 1; Sema3a, sema domain, secreted, (semaphorin) 3A; Spp1, secreted phosphoprotein 1, osteopontin; Smad2, MAD homolog 2 (Drosophila); Smad3, MAD homolog 3 (Drosophila); Mapk13, mitogen activated protein kinase 13 (p38); Hdac2, histone deacetylase 2; Trrap, transformation/transcription domain-associated protein; Hdac4, histone deacetylase 4; Mapk11, mitogen-activated protein kinase 11 (p38 δ); Tgfbrap1, transforming growth factor, beta receptor associated protein 1; Nfatc3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; Hdac3, histone deacetylase 3; Hdac10, histone deacetylase 10; Acvr2b, activin receptor IIB; Endogl1, endoglin (CD105);

ISSN: 1465-993X