Figure 3
Sorted cells retain cell surface markers typically associated with multipotent stromal cells. The CD34-, CD45-negative, Stro-1-, CD73-, CD105-positive cell fraction was plated, grown to confluence, passaged three times, removed with cell dissociation buffer, and bound to either AF488-conjugated control Ig (open curve) or antibody to the indicated antigen (grey curve). Cells in G0/G1 phase were gated by staining with propidium iodide. Event number is indicated on the y-axis.