Figure 2

OVA treatment increases the fraction of mesencymal stromal cell antigen-positive lung cells. Collagenase dispersible cells were prepared from the lungs of PBS- (left column) or OVA-treated mice (right column). Single cell suspensions from PBS or OVA-treated mouse lungs were allowed to adhere to polystyrene plates for 5 days, trypsinized, blocked with 1% BSA and incubated with AF350-conjugated anti-Stro-1, AF488 anti-CD34, AF750 anti-CD45, AF555 anti-CD105, and AF633 anti-CD73. Data from 100,000 events are shown, with Stro-1 fluorescence intensity in blue, CD73 in red and CD105 in green. Gates were drawn to select a Stro-1-positive (A), low CD34 (B) and low CD45 population (C). In panel D, the gates indicated in panels A, B and C were used to produce the CD73 vs. CD105 plots shown (dot size is adjusted to make the lower cell numbers visible). The average number of cells in the various CD73-, CD105-positive subpopulations shown is provided Table 1. There was a significant increase in the number of CD34-, CD45-negative, CD73-, CD105-positive cells in lungs from OVA-treated mice.