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Figure 1 | Respiratory Research

Figure 1

From: The early asthmatic response is associated with glycolysis, calcium binding and mitochondria activity as revealed by proteomic analysis in rats

Figure 1

Protocol and measurements for the rat model of allergic asthma. Male SD rats were sensitized intraperitoneally at day 0 with 1 mg of OVA precipitated with 10 mg of aluminum hydroxide gel in 1 ml saline. On day 14, rats were anaesthetized with 1% sodium pentobarbitone (wt/vol) at a dose of 50 mg/kg and challenged with 1 ml/kg of 5% OVA in normal saline (5 mg/kg) by injection into the external jugular vein over 10 s. The pulmonary resistance (RL), dynamic compliance (Cdyn) and respiratory rate (RR) were recorded for 10 min before challenge as the baseline values and then immediately measured for another 10 min after the challenge. The changes in the RL, Cdyn and RR were calculated as the differential values subtracted from the corresponding baseline values. The rats were euthanized immediately after the measurements were taken. Control rats were sensitized and challenged with saline instead of OVA.

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Respiratory Research

ISSN: 1465-993X

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