Effects of 'classical' polarization conditions on accumulation of T cell subsets. PBL from control and asthmatic subjects were cultured 5-d with CD3+CD28 monoclonal antibody + IL-2 and IL-4 + anti-IL-12 (A), IL-12 (B, C), or anti-IL-12 (C), and then stimulated and analyzed for cytokine production. Each symbol indicates the proportion (%) and number (n) of IL-13+ (A) and IFN-γ+ (B) T cells, expressed as a percent of respective the day 0 values for each subject tested. (C) Indicated are % and n of IFN-γ+ T cells in the IL-12 condition, expressed as a percent of respective anti-IL-12 (control) condition values. Bars = geometric mean for each parameter. p-values from student t-tests for differences in geometric mean between control and asthmatic subjects are shown.