Figure 1

Schematic demonstrating treatment and time course of measurements. Cells slated to undergo scrape and compression were scraped with a 200 μl pipette tip at time zero and washed 2× with cell culture media to remove debris and were imaged. The wells then underwent 30 cmH₂O mechanical compression for 6 hours. Following compression the cells were imaged and a representative well was fixed for imaging. 24 and 48 hours following the initial insult cells were again imaged to determine wound width, a representative well was fixed for imaging and media samples were taken for mediator content.