Figure 2

Endotoxin induced airway neutrophilia model. Rats were challenged with aerosolised endotoxin free saline or aerosolised LPS (0.3 mg/ml) for 30 minutes. BAL samples were taken at each time point for determination of A: neutrophil levels (eosinophils and LMN levels are shown in Table 2). B: Effect of an IKK-2 inhibitor (TPCA-1) or budesonide on BAL neutrophil levels. C: Levels of p65:DNA binding in the lung tissue. Statistical significance was determined using an unpaired t test with each independent group compared to the vehicle control. If the variances of the two groups were significantly different then the Mann Whitney rank sum test was used. A p-value of less than 0.05 was taken as significant and denoted with *. All the values are expressed as mean ± s.e. mean of 6 observations.