Figure 4

Effect of pretreatment with SNP (100 μM, n = 4, grey) and NCX 2057 (100 μM, n = 4, black) compared to control (Tyrode's solution, n = 4, white) on mediator release from sensitized GPLP after cumulative challenge with OVA. A, Histamine (ng/ml). B, CysLTs (pg/ml). All samples were collected at baseline and then after 10 min at the plateau of cumulative addition of 1, 10, 100 and 1000 ng/ml OVA. All data are expressed as mean ± s.e.m.; statistical analysis was performed by two-way ANOVA.*P < 0.05; ***P < 0.001 vs control.