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Figure 1 | Respiratory Research

Figure 1

From: A multi-target antisense approach against PDE4 and PDE7 reduces smoke-induced lung inflammation in mice

Figure 1

Effect of 4B/4D and 7A antisense oligonucleotides (AON) on target mRNA expression in NHBE cells. NHBE cells were transfected for 24 h with AON 4B/4D, with AON 7A, or with control sequences (CTRL) at 67, 134 or 267 nM, or were not transfected (NT). 4 h before the end of the transfection period, fresh media containing TNF-α, IL-1β and IFN-γ was added. mRNA levels for (A) PDE4B, (B) PDE4D and (C) PDE7A were quantified by real-time PCR analysis and normalized to a reference gene (Ppib). Results are expressed as percentage of PDE mRNA levels in non-transfected cells (± SEM). *: p < 0.05; **: p < 0.01, ***: p < 0.001, t-test relative to CTRL (n = 3 to 9 replicates).

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Respiratory Research

ISSN: 1465-993X

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