Adult onset lung disease following transient disruption of fetal stretch-induced differentiation

Respiratory Research

Table 1 Effect of in utero ASCFTR on lung parenchyma.

Age (days)	Group	% Parenchyma	% Airspace	A/P	Lm
13	Control (eGFP)	0.18956612	0.712707	3.787066	18.8125
13	ASCFTR	0.24464738	0.673135	2.806436	20.99217
		p = .003	p = .03	p = .004	p = .012
60	Control (eGFP)	0.12055785	0.773347	6.508026	19.15
60	ASCFTR	0.12007576	0.778168	6.862095	20.2
		NS	NS	NS	NS
90	Control (eGFP)	0.19871534	0.790174	4.622078	21.62444
90	ASCFTR	0.21126822	0.765983	4.568619	19.96945
		NS	NS	NS	NS
120	Control (eGFP)	0.16403409	0.850143	5.70228	18.405
120	ASCFTR	0.16472577	0.880894	5.84488	18.14
		NS	NS	NS	NS
210	Control (eGFP)	0.14146006	0.802755	6.003154	18.54375
210	ASCFTR	0.1651343	0.771823	4.979818	18.59688
		NS	NS	NS	NS

Detailed morphometric examination of the parenchyma at each age was performed by point counting at × 400 final magnification. Twenty images were examined from upper, middle and lower sections of each left lung following fixed-inflation. Volume densities of airspace wall, airspace and inter-airspace wall difference were determined by point counting. Inter-airspace wall difference (mean linear intercept (Lm)) was determined by counting the number of intercepts of a line of known length.

ISSN: 1465-993X