Figure 2

CFTR expression in DC from Cftr ^tm1UNC mice. RNA was extracted from WT and Cftr ^tm1UNC (CF) DC. CFTR expression was measured by real-time RT-PCR and reverse-transcription PCR. A. Real-time RT-PCR. Relative expression levels in the samples were calculated using the ΔΔCt method, using GAPDH as internal normalization control. The y-axis represents CFTR cDNA transcription level in terms of relative quantity value (RQ). B. Reverse-transcription PCR of CFTR in DC from WT and CF mice. Lung from WT mice were used as positive control. Primers were designed to detect WT CFTR cDNA but not mutant CFTR. GAPDH was used as endogenous PCR control. Shown is the mean ± SEM of three different samples. *denotes p < 0.05.