Figure 5

Effects of RGDS on FAK phosphorylation in lung tissue (A, B), and fibrinogen binding activity in alveolar macrophages (C, D). Where indicated, mice were administered RGDS or RGES (5 mg/kg, i.p.) once 1 h before LPS treatment and sacrificed 4 h post-LPS. Mice were also administered this peptide (5 mg/kg, i.p.) once 1 h before or 2 h after LPS and sacrificed 24 h post-LPS. (A, B) Western blotting with each anti-specific (phospho) FAK Ab was performed on lung tissue homogenates. Relative values for phosphorylated FAK versus FAK are indicated below the gel. Values represent means ± SEM of 5 mice per group. *Significantly different from saline treated controls, p < 0.05; ⁺significantly different from animals treated with LPS only, p < 0.05. (C, D) Alveolar macrophages were incubated with fibrinogen conjugated to Alexafluor-488 for 20 min. After exposure to fibrinogen for 20 min, cells were fixed and then analyzed by confocal fluorescence microscopy. The results are representative results obtained from 5 mice per group.