Figure 4

Effects of RGDS on LPS-induced TNF-α and MIP-2 productions, and on MMP-9 activity. Where indicated, mice were administered i.p. RGDS (1, 2.5 or 5 mg/kg) or RGES (5 mg/kg) once 1 h before LPS treatment and sacrificed 4 h post-LPS. Mice were also administered these peptides (5 mg/kg, i.p.) once 1 h before or 2 h after LPS and were sacrificed 24 h post-LPS (B). TNF-α and MIP-2 levels in BAL fluid samples were quantified using enzyme-linked immunosorbent assays (A, B, D). Gelatinolytic activity in BAL fluid samples (C, E). Samples were analyzed by zymography followed by scanning densitometry. The 92-kD genolytic bands corresponded to MMP-9. Densities are expressed as percentages versus saline treated controls. Values represent means ± SEM of results from 5 mice per group. *Significantly different from saline treated controls, p < 0.05; ⁺significantly different from animals treated with LPS only, p < 0.05.