HSP70 mRNA localization. (A) HSP70 mRNA was localized to the bronchial epithelium and parenchyma in unventilated controls. (B-E) Ventilation decreased HSP70 mRNA in airway epithelial cells and parenchyma. Injurious ventilation induced HSP70 mRNA in the smooth muscle surrounding large airways in all groups except the Beta group (C). (D) RNase protection assay for HSP70 mRNA in lung demonstrates decrease in lung mRNA with ventilation in all groups. *p < 0.05 vs controls.