Figure 8
From: Generation of lung epithelial-like tissue from human embryonic stem cells
Immunofluorescence double staining for SP-A and SP-C. hESC (VUB03_DM1) were cultured with the ALI differentiation protocol for 20 days. Original magnification 600×. Nuclear DAPI staining in blue. Staining for SP-A (red) and SP-C (green) were combined. SP-A was found on one side of the cells, while SP-C can be seen in the cytoplasm. Similar findings were obtained for VUB07 (not shown).