Figure 7
TGFβ 1 induces the expression of EMT-marker proteins and matrix-metalloproteinases in NHBE. A: NHBE cells were stimulated with TGFβ1 (2 ng/ml) or complete medium alone (control) for one to 3 days in triplicate. The levels of fibronectin-EDA, tenascin C, collagen I and vimentin mRNA were analyzed by quantitative real-time PCR as described in figure 5A. Results are representative of experiments performed with 2 different donors. (*p < 0.05 compared to control by Mann-Whitney U test) B: Conditioned supernatant of NHBE cells stimulated with TGFβ1 (5 ng/ml or 1 ng/ml) or complete medium alone were subject to Zymography. Results are representative of 2 separate experiments performed with 2 different donors.