Fig. 6

Curdlan increase but wedelolactone downregulate neutrophil related chemokine CXCL1 in HDM-induced MH-S cells. A, B mRNA expression of CXCL1and CXCR2 in the lungs of mice by qRT-PCR. MH-S cells were stimulated with PBS (50 μg/ml), HDM (50 μg/ml), HDM plus Laminarin (Lam)(1 μg/ml), HDM plus Curdlan (Cur) (200 μg/ml), or giving wedelolactone (Wed) (30 μM) after HDM/Curdlan for 24 h. CXCL1 (C), CXCL3 (D), CXCL5 (E), CXCL15 (F), G-CSF (G) and CXCL2 (H) mRNA expression levels in each group by qRT-PCR. (*P < 0.05; **P < 0.01;***P < 0.005; ****P < 0.001, one way ANOVA, error bars represent mean ± SEM)