Fig. 4

Activation of Dectin-1 promotes pyroptosis and pro-inflammatory function in MH-S cells. A The mean fluorescence intensity (MFI) of Dectin-1 on inflammatory cells in BALF of mice in each group by flow cytometry. MH-S cells were cultured in vitro, stimulated with PBS (50 μg/ml), HDM (50 μg/ml), HDM plus Curdlan (200 μg/ml) or HDM plus Laminarin(1 μg/ml) for 24 h. B, C The MFI of Dectin-1 expression on MH-S cells in each stimulation group by flow cytometry. D Optical microscope images of cell morphology in each stimulation group. E LDH release in supernatant of cells in each stimulation group. F The mRNA expression level of iNOS, TNF-α, Arg-1, Fizz1 in each stimulation group by qRT-PCR. (*P < 0.05; **P < 0.01;***P < 0.005; ****P < 0.001, one way ANOVA, error bars represent mean ± SEM)