Fig. 3

Caspase-11 inhibitor has therapeutic effect on Curdlan-induced asthma mice. C57BL/6 mice were divided into 4 groups, HDM, HDM + Wed (wedelolactone), HDM + Cur (Curdlan) and HDM + Cur + Wed group. Wedelolactone (20 mg/kg, 200 μl) was administered to mice before stimulation with HDM or HDM/Curdlan at days 0, 2, 4, 11 to 14. A The lung tissue of each group was stained with H&E and PAS (25x). B, C Inflammatory score of lung histopathology by H&E staining and PAS staining. D–H The counts of total cells and eosinophils, lymphocytes, neutrophils and macrophages and in BALF of each mice group were detected by flow cytometry. I–L mRNA expression of IL-1α, IL-1β, IL-6 and IL-17a in the lungs of mice by qRT-PCR. (n = 4–8). (*P < 0.05; **P < 0.01;***P < 0.005; ****P < 0.001, one way ANOVA, error bars represent mean ± SEM) Wed: wedelolactone