Fig. 2

Caspase-11 and pyroptosis is activated in Dectin-1 agonist induced asthma mice. A Expression of pro caspase-11 and cleavage caspase-11 in mice lung tissue by western blot. B Densitometric quantification of immunoblots in (A). Levels of cleavage caspase-11 were normalized to β-tubulin. C Densitometric quantification of immunoblots in (D). D Expression of full length of gasdermin D (GSDMD-FL) and N-terminal fragments of gasdermin D (GSDMD-N) in mice lung tissue by western blot. B Levels of GSDMD-N were normalized to β-actin. E, F IL-1α and IL-1β mRNA expression in lung tissue of mice by qRT-PCR. (n = 4–6). (*P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001, one way ANOVA, error bars represent mean ± SEM)