Fig. 1

Macrophage polarization occurs in in vivo sepsis models. (A) Lung tissue was analyzed by H&E staining, and the expression levels of iNOS, CD206, MPO, and SOD were analyzed by IHC, bar = 50 μm. (B) The M1 marker (iNOS) and the M2 marker (CD206) were measured in mice which treated with saline or LPS. (C) Relative macrophage polarization related protein expression of M1 marker (iNOS) and M2 marker (CD206) in CLP mice or wild-type mice. (D) Quantitative RT-PCR analysis of the M1 markers and the M2 markers in mice treated with LPS. (E) Quantitative RT-PCR analysis of the M1 markers and the M2 markers in CLP model. (F, G) The expression of CD68 in lungs was examined by immunohistochemistry and iNOS, and CD206 level in macrophages (CD68-positive cells) were detected by immunofluorescence. (200×: bar = 50 μm, 400×: bar = 20 μm). ^*P < 0.05 vs the control group. Ns, not significance