Fig. 6

The MAPK signaling pathway may be involved in the miR-23a-5p/RAGE regulatory module in the pathogenesis of COPD. A, B Western Blotting detection of ERK phosphorylation levels in lung tissues of different groups of mice (n = 3); C, D Western Blotting detection of ERK phosphorylation levels in HBE cells transfected with miR-23a-5p mimics; E, F rt-PCR detection of mRNA levels of IL-6 and IL-8 in HBE cells after ERK inhibitor administration; G-H: ELISA detection of IL-6 and IL-8 secretion levels in HBE cells after ERK inhibitor (PD98059) administration. The experimental results were independently repeated three or more times, and Data are least squares means ± standard errors. *P < 0.05, **P < 0.01, ***p < 0.001