Fig. 1
From: Deciphering the molecular regulatory of RAB32/GPRC5A axis in chronic obstructive pulmonary disease
Identification of key Rab genes associated with COPD pathogenesis using WGCNA. (A) Evaluation of scale-free fit index and mean connectivity at different soft threshold powers. (B) Heatmap illustrating the correlation between module eigengenes and COPD status. Each cell displays the respective correlation coefficient and P-value. (C) Inter-correlation among WGCNA module eigengenes. (D) Scatter plot depicting the correlation between module membership within the MEgreenyellow module and Gene significance. (E) Venn diagram highlighting the intersection between WGCNA hub genes and the Rab GTPase family. (F) Clinical pathological parameters of ten lung tissues samples. (G) Bar plot demonstrating the difference in FEV1/FVC% between non-COPD and COPD groups (n = 5). (H) RT-qPCR analysis revealing mRNA expression differences of hub members within the Rab GTPase family between non-COPD and COPD groups (n = 5). (I) Western blotting and its quantification of RAB32 protein levels in lung tissues of non-COPD and COPD groups (n = 5). Note: ns denotes non-significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001