Fig. 2

Composition of immune lineage cells differ in EVALI compared to otherwise healthy e-cigarette controls. Flow cytometric analysis was completed using a macrophage specific flow panel that included a viability dye, and antibodies specific for CD45, CD11c, CD11b, CD14, CD16, CD206, CD64 and CD163. (A)-(C) Total immune cells were detected as CD45 + cells in three groups healthy participants (A; healthy), e-cigarette controls (B; e-Cig), and EVALI subjects (C; EVALI). (D) The counts of CD45 + cells were normalized per mL of returned lavage fluid and shown as count/mL. (G-I) gating schematic and counts of (E) dendritic cells and (F) lymphocytes per mL of returned BAL fluid. (M-O) quantitation for (M) CD16^lo and (N) CD16^high inflammatory monocytes and (O) neutrophils and (J-L) the representative flow gating for each group. (J-L) shows the representative gating for alveolar macrophages, populations are contained in the dark red box. The quantitation of (P) total alveolar macrophages (aMacs), (Q) CD163- M1 macrophages and (R) CD163 + M2 macrophages. The composition of total BAL of each of the above cell populations are displayed in S-U for each group. Statistical significance was determined using an ordinary One-Way ANOVA with Dunnett’s post-test to determine between groups differences; *indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001. Sample size is n = 7 for healthy controls, n = 13 for e-Cig controls and n = 10 for EVALI subjects