Fig. 5

Functionality of inositol in IPF lung fibroblasts. A Effect of inositol exposure on the colony-forming ability of normal and IPF primary lung fibroblasts. Cells were seeded to grow and treated with indicated concentration of inositol for 14 days. Left: representative images of cell colonies. Right: quantification of colonies stained by crystal violet and counted under phase microscopy (mean ± SE, n = 3). B Cell viability of IPF lung fibroblasts treated with inositol. IPF lung fibroblasts were incubated in various concentrations of inositol for 72 h, and cell viability was assessed by a CCK8 assay (mean ± SE, n = 3). C Analysis of cell invasion ability of inositol-treated IPF lung fibroblasts determined by Matrigel invasion assays. Left: representative images of stained invaded cells. Right: quantification of invaded cell number (mean ± SE, n = 3, *p < 0.05 versus untreated group)