Fig. 4
From: PEEP application during mechanical ventilation contributes to fibrosis in the diaphragm
Application of PEEP during mechanical ventilation leads to collagen deposition in the diaphragm. A–C Sirius red staining with bright field and polarized microscopy revealed increased collagen fibers in the diaphragms of the MV + PEEP group. Scale bar = 50 μm. D–H Diaphragm protein lysates of the three groups were detected by Western blotting, and the expression of different collagen types was quantified. I, J Diaphragmatic tissue sections were subjected to immunofluorescence staining to analyze the expression of collagen III. Red represents collagen III; green represents WGA; blue represents Hoechst. Scale bar = 50 μm. Values are represented as the mean ± SD. n = 6 (*p < 0.05, **p < 0.01, ***p < 0.001)