Fig. 7

Neutrophil extracellular trap production by peripheral blood and sputum neutrophils in PCD and bronchiectasis. Peripheral blood neutrophils from patients with PCD (PCD blood, n = 15) and non-PCD, non-CF bronchiectasis (BE blood, n = 4) and purified sputum cells from patients with PCD (PCD sputum, n = 5) and non-PCD, non-CF bronchiectasis (BE sputum, n = 4) were exposed to RPMI medium or PMA for 3 h. Using SYTOX Green labeling of extracellular DNA, the degree of NETosis was calculated. A Representative microscopy pictures of peripheral blood neutrophils and purified sputum cells at baseline (0 h) and after 3 h of stimulation with PMA. The scalebar is 200 µm. B The amount of DNA detectable prior to stimulation. Results are displayed as the green fluorescent area normalized to the cell area. C The amount of DNA detectable after exposing the cells for 3 h to PMA. Results are displayed as fold increase in green fluorescent area relative to the unstimulated condition